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FAQ

General

We offer two different systems, which we call Starter kits, to be used with the sensors. Each Starter kit consists of a tablet with software, 4 beamers (to transmit the signal from the sensor to the tablet) and only for CITSens Bio a radio receiver. 1) With CITSens Bio you can measure glucose or lactate with one sensor and one transmitter 2) With CITSens MeMo you can measure both glucose and lactate with one sensor and one transmitter. For both systems an automated process control software upgrade (APC) is available (at the moment with micropumps or Ismatec peristaltic pump Reglo Digital MS4/6) so that you can do automated media feed based on in-situ measured glucose concentration. Our sensors can be integrated into any caps (T-Flasks, Cell Stacks, Shake Flasks, etc.) or are also available as pg13.5 process probe for SUBs as well as flow cells for perfusion systems. Integration of our probes into a stainless steel (SS) bioreactor is possible with a quick connection coupling from e.g. CPC (one part in situ sterilizable, one part gamma irradiated)
- Results at any time and everywhere available - No additional sampling necessary - Reduced risk of contamination - No reagents-cost compared to Offline methods - Immediate recognition of contamination and other process-changes - No falsification of the sample due to sampling
CITSens Bio and CITSens MeMo are the first and only systems for online, in situ measurement of glucose and lactate. The sensor's application fields are Regenerative medicine, Bioprocessing, Cell and Gene Therapy as well as medical applications.
Each sensor is gamma-irradiated (25 kGY) and double packed.
No, the enzyme will immediately denature due to the heat of the autoclaving process. But sensors are delivered gamma-irradiated (25 kGY) and double packed. Integration of our probes into a stainless steel (SS) bioreactor is possible with a quick connection coupling from e.g. CPC (one part in situ sterilizable, the other gamma irradiated).
With CITSens Bio one parameter with each sensor (Glucose sensor and Lactate sensor), with CITSens MeMo two parameters with one sensor (Glucose and Lactate sensor).
Depending on the reactor type: - Single Use Bioreactors: depending on available PG13.5 ports - Shake-, T- Flasks: 2 sensors per lid
It depends on the bioreactor shape. Always make sure that the sensing part of the sensor is fully wetted. We usually recommend a minimum working volume of 5 mL (e.g. 6 well plate)
The Glucose sensor is very specific and detects only the β-D-glucose. The Lactate sensor is very specific and detects only L-Lactate.
No, because the paste on the sensor is covered and sealed with a membrane or polymer with a pore size of 6 kD which excludes all large molecules from diffusion. The paste never gets into direct contact with the medium and the cells.
Depending on the cell-type and growth behavior cells might adhere. Even tough this generally does not affect the sensor's performance.
The sensors last up to 3 weeks in process. Depending on the media and process strategy the lifetime of the sensor may vary.
The sensors are stable during 6 months in the fridge at 4 °C.
This is possible, nevertheless, C-CIT Sensors AG does not recommend nor take over any responsibility regarding the sterility if the sensor was transferred.
Yes. The Stop/Pause button in the software can be used therefore.
Generally the sensor should only be calibrated at the beginning of an experiment. In case of a temperature or pH shift during the process re-calibration might be necessary.
The software has a status announcement in the “calibrate sensors” menu where the current calibration status of the sensor is indicated.
The sensor in the medium produces electrons which must be led away. If this is not the case, peroxide formation takes place which will affect the enzyme and thereby the sensor performance. Short disconnecting (<5 min) does not affect the sensor. Even though it is not recommended to disconnect the sensor at any time during the process.
The ID-tag of the Beamer appears in the software as soon as a sensor is connected to the Beamer. To figure out if the Beamer works during process please enter the software and enter the “Measurement” menu. Choose the Beamer and click on “last 30 measured values”. If the measured data is updated according to the present time indicated at the computer the Beamer runs properly.
Yes. Generally the values do not deviate more than 10% from Offline reading. In addition it is very important to determine the right moment of taking Offline samples. In case comparability studies are taking place it is recommended to wait until the sensor signal has stabilized specially after a feed before taking a sample for Offline measurement.
The CITSens sensors were compared to HPLC, CEDEX, Bioprofile
It is calculated automatically by the software. In case the calibration did not take place during the process the data can be calibrated in Excel afterwards.
The measured values can be processed with any program (e.g. Excel, Word, etc.). It is IMPORTANT to IMPORT the exported .csv file to Excel. Otherwise splitting of the data is not possible and all values will be shown in one column.
We provide 3 different ways to integrate our data into customer's process control devices: 1. Integration of our CITSens Bio or MeMo system into your process control via LAN This is the easiest option to incorporate the sensor reading of our system into any other pcs. Usually this is done by connecting our PC to your control system via LAN cable (peer to peer). Using an OPC client will then allow to extract data from our mySQL data base and include those data in your pcs as a soft sensor. Additionally this option allows to run the system as a stand alone unit in order to have a first hands on experience and easy evaluation possibility. 2. Integration of the Zomofi receiver into your process control device We will provide you the necessary communication protocols but need some IT work on your site. The sensors still need our standard beamers. 3. Analog module (transmitter) There is a possibility to extract the raw signal from our electronics and convert it into a 4-20mA, respectively 0-10mV signal. To realize this option we need to know what kind of plug and analog input you are using at your system.

CITSens Bio

No, the enzyme which is immobilized on the sensor generates a current which is collected and proceeded by the beamer. Additionally the beamer is responsible for the communication with the ZOMOFI receiver. The ZOMOFI receiver is connected to the system PC and stores each data point in the MySQL data base.
A green light on the back of the ZOMOFI flashes approx. every 20 sec. Additionally the connection status can be determined by entering the “Server setup” → “ZOMOFI setup” menu in the software. If the controller status is ON it means that the ZOMOFI is connected and running
One ZOMOFI can handle up to 200 Beamers in parallel.
The maximum distance is at around 20 meters. Depending on the environment the range can be decreased (Incubator, wall, etc.)

CITSens MeMo

One Tablet integrated receiver can handle up to 12 Beamers in parallel.
The maximum distance is at around 10 meters. Depending on the environment the range can be decreased (Incubator, wall, etc.)

CITSens Bio APC & CITSens MeMo APC

CITSensBio APC and CITSens MeMo APC are like the standard CITSens Bio and CITSens MeMo, but they have an additional software upgrade to control the pumps in order to do automated process control based on glucose concentration
CITSens Bio APC can be connected to 24x Reglo Digital MS-4/6 pumps or to 12x micropumps. CITSens MeMo APC can be connected to 12x Reglo Digital MS-4/6 pumps or to 12x micropumps. It is also possible to connect other RS232 pumps, it just requires some extra customized communication programming