- Results at any time and everywhere available - No additional sampling necessary - Reduced risk of contamination - No reagents-cost compared to Offline methods - Immediate recognition of contamination and other process-changes - No falsification of the sample due to sampling
CITSens Bio and CITSens MeMo are the first and only systems for online, in situ measurement of glucose and lactate. They can be used in Cell Culture Technology, Microbial Fermentation and Blood
No, the enzyme which is immobilized on the sensor generates a current which is collected and proceeded by the beamer. Additionally the beamer is responsible for the communication with the ZOMOFI receiver. The ZOMOFI receiver is connected to the system PC and stores each data point in the MySQL data base.
Each sensor is gamma-irradiated (25 kGY) and double packed.
No, the enzyme will immediately denature due to the heat of the autoclaving process.
With CITSens Bio one parameter with each sensor (Glucose sensor and Lactate sensor), with CITSens MeMo two parameters with one sensor (Glucose and Lactate sensor).
Depending on the reactor type: - Single Use Bioreactors: depending on available PG13.5 ports - Shake-, T- Flasks: 2 sensors per lid
It depends on the bioreactor shape. Always make sure that the sensing part of the sensor is fully wetted.
The Glucose sensor is very specific and detects only the β-D-glucose. The Lactate sensor is very specific and detects only L-Lactate.
No, because the paste on the sensor is covered and sealed with a membrane or polymer with a pore size of 6 kD which excludes all large molecules from diffusion. The paste never gets into direct contact with the medium and the cells.
Depending on the cell-type and growth behavior cells might adhere. Even tough this generally does not affect the sensor's performance.
The sensors last up to 3 weeks in process. Depending on the media and process strategy the lifetime of the sensor may vary.
The sensors are stable during 6 months in the fridge at 5 °C.
This is possible, nevertheless, C-CIT Sensors AG does not recommend nor take over any responsibility regarding the sterility if the sensor was transferred.
Yes. The Stop/Pause button in the CITSens Bio software can be used therefore.
Generally the sensor should only be calibrated at the beginning of an experiment. In case of a temperature or pH shift during the process re-calibration might be necessary.
The CITSens Bio software has a status announcement in the “calibrate sensors” menu where the current calibration status of the sensor is indicated.
The sensor in the medium produces electrons which must be led away. If this is not the case, peroxide formation takes place which will affect the enzyme and thereby the sensor performance. Short disconnecting (<5 min) does not affect the sensor. Even though it is not recommended to disconnect the sensor at any time during the process.
The ID-tag of the Beamer appears in the software as soon as a sensor is connected to the Beamer. To figure out if the Beamer works during process please enter the CITSens Bio software and enter the “Measurement” menu. Choose the Beamer and click on “last 30 measured values”. If the measured data is updated according to the present time indicated at the computer the Beamer runs properly.
A green light on the back of the ZOMOFI flashes approx. every 20 sec. Additionally the connection status can be determined by entering the “Server setup” → “ZOMOFI setup” menu in the CITSens Bio software. If the controller status is ON it means that the ZOMOFI is connected and running
One ZOMOFI can handle up to 200 Beamers in parallel.
The maximum distance is at around 20 meters. Depending on the environment the range can be decreased (Incubator, wall, etc.)
Yes. Generally the values do not deviate more than 10% from Offline reading. In addition it is very important to determine the right moment of taking Offline samples. In case comparability studies are taking place it is recommended to wait until the sensor signal has stabilized specially after a feed before taking a sample for Offline measurement.
It is calculated automatically by the CITSens Bio software. In case the calibration did not take place during the process the data can be calibrated in Excel afterwards.
The CITSens sensors were compared to HPLC, CEDEX, Bioprofile
The measured values can be processed with any program (e.g. Excel, Word, etc.). It is IMPORTANT to IMPORT the exported .csv file to Excel. Otherwise splitting of the data is not possible and all values will be shown in one column.