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FAQ

General information

Each of our Glucose &/or Lactate sensor needs a transmitter to send the data to a Tablet on which the Software/s to monitor/and control is/are installed. Transmitters and Tablet with software/s are part of a Starter Kit. CITSens Bio, CITSens Bio APC, CITSens MeMo Basic Version and CITSens MeMo Full Version are the Starter Kits we offer. Our sensors can be integrated into any caps (T-Flasks, Cell Stacks, Shake Flasks, G-Rex M Series, etc.) or are also available as pg13.5 process probe for SUBs as well as flow cells for perfusion systems or flow cells with micropump for Wave Bags. Integration of our probes into a stainless steel (SS) bioreactor is possible with a quick connection coupling from e.g. CPC (one part in situ sterilizable, one part gamma irradiated)
- Results at any time and everywhere available - No additional sampling necessary - Reduced risk of contamination - No reagents-cost compared to Offline methods - Immediate recognition of contamination and other process-changes - No falsification of the sample due to sampling
CITSens Bio APC can be connected to 24x Reglo Digital MS-4/6 pumps or to 12x micropumps. CITSens MeMo Full Version can be connected to 12x Reglo Digital MS-4/6 pumps or to 12x micropumps. It is also possible to connect other RS232 pumps, it just requires some extra customized communication programming
CITSens Bio, CITSens Bio APC, CITSens MeMo Basic Version and CITSens MeMo Full Version are the first and only systems for online, in situ measurement of glucose and lactate. The sensor's application fields are Regenerative medicine, Bioprocessing, Cell and Gene Therapy as well as medical applications.
Each sensor is gamma-irradiated (25 kGY) and double packed.
No, the enzyme will immediately denature due to the heat of the autoclaving process. But sensors are delivered gamma-irradiated (25 kGY) and double packed. Integration of our probes into a stainless steel (SS) bioreactor is possible with a quick connection coupling from e.g. CPC (one part in situ sterilizable, the other gamma irradiated).
With CITSens Bio and CITSens Bio APC one parameter with each sensor (Glucose sensor and Lactate sensor), with CITSens MeMo Basic and Full Version whether one or two parameters with one sensor (Glucose and Lactate sensor).
Depending on the reactor type: - Single Use Bioreactors: depending on available PG13.5 ports - Shake-, T- Flasks: 2 sensors per lid
It depends on the bioreactor shape. Always make sure that the sensing part of the sensor is fully wetted. We usually recommend a minimum working volume of 5 mL (e.g. 6 well plate)
The Glucose sensor is very specific and detects only the β-D-glucose. The Lactate sensor is very specific and detects only L-Lactate.
No, because the paste on the sensor is covered and sealed with a membrane or polymer with a pore size of 6 kD which excludes all large molecules from diffusion. The paste never gets into direct contact with the medium and the cells.
Depending on the cell-type and growth behavior cells might adhere. Even tough this generally does not affect the sensor's performance.
The sensors last up to 3 weeks in process. Depending on the media and process strategy the lifetime of the sensor may vary.
The sensors are stable during 6 months in the fridge at 4 °C.
This is possible, nevertheless, C-CIT Sensors AG does not recommend nor take over any responsibility regarding the sterility if the sensor was transferred.
Yes. The Stop/Pause button in the software can be used therefore.
Generally the sensor should only be calibrated at the beginning of an experiment. In case of a temperature or pH shift during the process re-calibration might be necessary.
The software has a status announcement in the “calibrate sensors” menu where the current calibration status of the sensor is indicated.
The sensor in the medium produces electrons which must be led away. If this is not the case, peroxide formation takes place which will affect the enzyme and thereby the sensor performance. Short disconnecting (<5 min) does not affect the sensor. Even though it is not recommended to disconnect the sensor at any time during the process.
The ID-tag of the transmitter appears in the software as soon as a sensor is connected to the transmitter. To figure out if the transmitter works during process please enter the software and enter the “Measurement” menu. Choose the transmitter and click on “last 30 measured values”. If the measured data is updated according to the present time indicated at the computer the transmitter runs properly.
Yes. Generally the values do not deviate more than 10% from Offline reading. In addition it is very important to determine the right moment of taking Offline samples. In case comparability studies are taking place it is recommended to wait until the sensor signal has stabilized specially after a feed before taking a sample for Offline measurement.
The CITSens sensors were compared to HPLC, CEDEX, Bioprofile
It is calculated automatically by the software. In case the calibration did not take place during the process the data can be calibrated in Excel afterwards.
The measured values can be processed with any program (e.g. Excel, Word, etc.). It is IMPORTANT to IMPORT the exported .csv file to Excel. Otherwise splitting of the data is not possible and all values will be shown in one column.
We provide 3 different ways to integrate our data into customer's process control devices: 1. Integration of our CITSens Bio or MeMo systems into your process control via LAN This is the easiest option to incorporate the sensor reading of our system into any other pcs. Usually this is done by connecting our PC to your control system via LAN cable (peer to peer). Using an OPC client will then allow to extract data from our mySQL data base and include those data in your pcs as a soft sensor. Additionally this option allows to run the system as a stand alone unit in order to have a first hands on experience and easy evaluation possibility. 2. Integration of the Zomofi receiver into your process control device We will provide you the necessary communication protocols but need some IT work on your site. The sensors still need our standard beamers. 3. the USB wired board can be connected to any computer supporting virtual COM Port connected by USB or RS232. Due to its modular design the USB wired Board supports amperometric as well as potentiometric measurements which allows to read out our glucose & lactate sensors as well as pH & NH4+ sensors. The USB wired Board supports 2 channel read out that allows measuring e.g. glucose & lactate simultaneously and is compatible with all the sensors in the portfolio

CITSens Bio and CITSens Bio APC

With CITSens Bio it is possible to monitor Glucose or Lactate. With CITSens Bio APC it is not only possible to monitor Glucose or Lactate but also to control the process automatically based on Glucose concentration
No, the enzyme which is immobilized on the sensor generates a current which is collected and proceeded by the transmitter. Additionally the transmitter is responsible for the communication with the ZOMOFI receiver. The ZOMOFI receiver is connected to the system PC and stores each data point in the MySQL data base.
A green light on the back of the ZOMOFI flashes approx. every 20 sec. Additionally the connection status can be determined by entering the “Server setup” → “ZOMOFI setup” menu in the software. If the controller status is ON it means that the ZOMOFI is connected and running
One ZOMOFI can handle up to 200 transmitters in parallel.
The maximum distance is at around 20 meters. Depending on the environment the range can be decreased (Incubator, wall, etc.)

CITSens MeMo Basic and Full Version

The CITSens MeMo Basic Version includes one tablet with monitoring software and one transmitter (whether bluetooth or wired). This version is limited to the use of one transmitter and has no automated process control (APC) software. It is possible to upgrade the Basic Version to a Full Version in a modular way, purchasing separately: – Tablet Upgrade for up to 12x transmitters – CITSens APC Software upgrade for automated process control – Further transmitters The CITSens MeMo Full Version includes one tablet with monitoring and automated process control (APC) softwares and four transmitters (whether bluetooth or wired). By purchasing additional transmitters it is possible to monitor and control twelve bioreactors simultaneously.
With CITSens MeMo Basic Version 1 transmitter. With CITSens MeMo Basic Version + Tablet Upgrade or CITSens MeMo Full Version one Tablet can handle up to 12 transmitters in parallel.
The maximum distance is at around 10 meters. Depending on the environment the range can be decreased (Incubator, wall, etc.)